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Koch postulate – Introduction, procedure,exception, classified disease, symptoms and limitations full overview
Koch postulate :-
- Koch postulate was define in 1876 german scientist Robert Koch. Scientist Koch cultivate to bacillus anthrax using of blood serum.
Introduction of Koch postulate :-
- The disease may be present in every died animal but absent in healthy individuals.
- The agent of Koch postulate is must be isolated and cultured in-vitro.
- Culture of this disease is cultivated in laboratory condition.
- Robert Koch discovered different disease and microorganisms such as tuberculosis mycobacterium, Vibrio cholera and Bacillus anthracis.
- Robert Koch play important role in microbiology and also known as father of bacteriology.
Procedure of Koch postulate :-
- Microorganisms are isolated from dead animals.
- Then organisms are grown in pure culture medium.
- After growing of organisms then organisms are identified.
- Later microorganisms are injected into the healthy animals.
- Then disease is produced in second animal.
- After we again grow microorganism on the pure culture medium.
- Then identification of microorganisms in culture medium.
Exception of Koch postulate :-
- Microorganisms that are not able to culture on artificial media E.g. Treponema pallidum.
- When two or more organisms are work together then they cause infection.
- Symptoms and diseases can be caused by any one of different organism.
- Not all the disease behave the same.
- Disease very widely in their duration, onset and level of activity.
Classified disease :-
- Acute disease :- Symptoms develop rapidly and tend to be more severe.
- Chronic disease :- Symptoms develop slowely and are less severe.
- Sub-acute disease :- Symptoms of both acute and chronic are present.
- Latent disease :- Disease with a period of no symptoms when the causative agent in active.
Symptoms in plants and humans :-
- Yellowing of leaves and spots on plant parts.
- Rotted plant parts and dead tissue.
- In human fever, headache and loose motion like symptoms are appear.
Limitation of Koch postulate :-
- Some microorganisms are cannot grow on the pure culture medium.
- Using humans in completing the postulates is unethical.
- Molecular and genetic evidence can be replace in postulate.
- Disease production can need co-factor.
- Always not possible to isolate organisms from disease person.
- Virus cannot be cultured like bacteria because living cell to grow.
- Always infection does not produce infection.
- Pathogen can be present without clinical disease.