In-vitro fertilization (IVF) -Introduction, Procedure and Advantages

In-vitro fertilization (IVF) :-

  • In-vitro fertilization is addition of union between egg cells and outside the body in culture vessels.

Introduction of In-vitro fertilization :-

  • IVF is involved in collection and combination of gametes under appropriate condition.
  • IVF is one type of In-vitro technique such as they take living organisms in test-tube, beakers and petridish.
  • The first test-tube baby or IVF baby was born in 1978.
  • In India first test-tube was born in same year in 1978 and this second test-tube baby of the world.
  • Dr. subhash mukhopadhyay was a physician from Kolkata India, who created world second and India‚Äôs first test-tube baby.
  • In 2010 sir Edwards was awarded won nobel prize for discovery In-vitro fertilization in physiology.
  • In vitro fertilization done for infertility treatment and problem in the fallopian tube of female and low sperm quality of male.

Procedure of In-vitro fertilization :-

In-vitro fertilization (IVF)
In-vitro fertilization (IVF)
  1. Ovarian stimulation :-
  • Leuprolide injection for control premature release eggs one week before.
  • Then follicle stimulating hormone (FSH) injection stimulate maturation multiple eggs.
  • After a single injection of human chronic gonadotropin (HCG) in last stage of maturation of eggs.
  1. Eggs retrivel :-
  • Eggs are retrivel after 36 hrs of human chronic gonadotropin (HCG) injection.
  • Then needle enters in ovarian follicle.
  • After we used microscopic technique for identification of eggs.
  1. Sperm collection :-
  • Then sperm collected from the partner.
  • Proceed and incubated in protein supplemented media for 3 to 4 hours.
  1. Fertilization in the laboratory :-
  • Sperm placed in culture with a single oocyte in a petridish.
  • Signs of fertilization and presence of 2-pronulei.
  1. Embryo culture :-
  • Take embryo culture from the tubes and transfer embryo.
  1. Embryo transfer :-
  • 4 to 7 cell embryo are transferred.
  • Cathter is instrument that used in transfer of embryo cells.
  • Then embryo released in upper part of uterus.
  • Not more then 3 embryo can be transferred.
  1. Cryopreservation :-
  • Preservation in frozen state is regarded as cryopreservation.
  • Embryo can be cryo-preserved in presence of glycerol.
  • It was stored at -196 celcius under liquid nitrogen.
  • And transferred to uterus.
  • And this is process of In-vitro fertilization (IVF).

Advantages :-

  • Fertilization is confirmed before implantation appear.
  • Give woman with defected oviducts, the opportunity to carry their own fetus.

Add a Comment

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.