Granulocyte colony stimulating factor (GCSF) :- Introduction and Forms

Granulocyte colony stimulating factor (GCSF) :-

  • Granulocyte colony stimulating factor is produced by endothelium, macrophage and other immune cells.

Introduction of Granulocyte colony stimulating factor (GCSF) :-

  • GCSF have two type of structural forms.
  • First structural form is made up of 174 amino acid and second structural form is made up of 180 amino acids.
  • GCSF was first discovered by Walter and Eliza Hall institute Australia in 1983.
  • GCSF was identified when scientists are testing Heamopoetic stem cells (HSCs) for colony stimulating factors.
  • Bone marrow, Stromal cells, Neutrophils, Monocytes, T cells and Endothelial cells are used in the Endogenous GCSF production.
  • Endogenous GCSF regulates the production, function of the neutrophil lineage and maturation.
  • Blood range of GCSF is 20 to 100 pg/ml.
  • Biological functions of Granulocyte colony stimulating factor (GCSF) :-
  • Granulocytes are initiated, proliferated and differentiated by the GCSF.
  • GCSF is one type of cytokine that has play very major role in the myeloid lineage of haematopoiesis.
  • Due to its demonstrated efficiency against various forms of netropenia and chemotherapy induced leucopenia.
  • As well as its suitability for allogeneic and autologous bone marrow transplantation and peripheral blood stem cell mobilization.
  • GCSF is widely used in the pharmaceutical and medical industries.
  • There are two form of natural Granulocyte colony stimulating factor (GCSF) that have been isolated from tumor cell lines.

    Granulocyte colony stimulating factor
    Granulocyte colony stimulating factor
  1. Short form :-

  • Short from is composed of 174 amino acids and more active then other one.
  1. Long form :-

  • Long form is composed more then of 3 amino acids.
  • Due to its pharmacological potential Granulocyte colony stimulating factors are already used in bacteria, yeast, mammalian cell line and plant cell cultures.
  • There are two forms of GCSF in clinical uses.
  1. Non-glycosylated :-

  • In this form Escherichia coli is used derived form.
  1. Glycosylated :-

  • CHO cell derived form that is indistinguishable from its natural counterpart.
  • Glycosylation of GCSF does not occur to be essential for its biological activity.
  • But it has been shown to be beneficial for its in vitro stability.
  • The O-linked glycosyl groups prevent the GCSF protein against molecular aggregation and protease degradation in human serum.

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