November 6, 2018 HomeHealth BiotechnologyGranulocyte colony stimulating factor (GCSF) :- Introduction and Forms Granulocyte colony stimulating factor (GCSF) :- Introduction and Forms By Jay Hind Health Biotechnology 0 Comments Granulocyte colony stimulating factor (GCSF) :- Granulocyte colony stimulating factor is produced by endothelium, macrophage and other immune cells. Introduction of Granulocyte colony stimulating factor (GCSF) :- GCSF have two type of structural forms. First structural form is made up of 174 amino acid and second structural form is made up of 180 amino acids. GCSF was first discovered by Walter and Eliza Hall institute Australia in 1983. GCSF was identified when scientists are testing Heamopoetic stem cells (HSCs) for colony stimulating factors. Bone marrow, Stromal cells, Neutrophils, Monocytes, T cells and Endothelial cells are used in the Endogenous GCSF production. Endogenous GCSF regulates the production, function of the neutrophil lineage and maturation. Blood range of GCSF is 20 to 100 pg/ml. Biological functions of Granulocyte colony stimulating factor (GCSF) :- Granulocytes are initiated, proliferated and differentiated by the GCSF. GCSF is one type of cytokine that has play very major role in the myeloid lineage of haematopoiesis. Due to its demonstrated efficiency against various forms of netropenia and chemotherapy induced leucopenia. As well as its suitability for allogeneic and autologous bone marrow transplantation and peripheral blood stem cell mobilization. GCSF is widely used in the pharmaceutical and medical industries. There are two form of natural Granulocyte colony stimulating factor (GCSF) that have been isolated from tumor cell lines. Granulocyte colony stimulating factor Short form :- Short from is composed of 174 amino acids and more active then other one. Long form :- Long form is composed more then of 3 amino acids. Due to its pharmacological potential Granulocyte colony stimulating factors are already used in bacteria, yeast, mammalian cell line and plant cell cultures. There are two forms of GCSF in clinical uses. Non-glycosylated :- In this form Escherichia coli is used derived form. Glycosylated :- CHO cell derived form that is indistinguishable from its natural counterpart. Glycosylation of GCSF does not occur to be essential for its biological activity. But it has been shown to be beneficial for its in vitro stability. The O-linked glycosyl groups prevent the GCSF protein against molecular aggregation and protease degradation in human serum. Tags:Granulocyte colony stimulating factor Related Posts Kuru disease : Introduction, symptoms, causes and treatment Duchenne Muscular Dystrophy :- Symptoms, Diagnosis and Treatment Thalassemia disorder : Types, symptoms, diagnosis and treatment Add a Comment Cancel reply Your email address will not be published. Required fields are marked *Comment:*Name:* Email Address:* Website: Save my name, email, and website in this browser for the next time I comment. This site uses Akismet to reduce spam. Learn how your comment data is processed.